The 2007–08 season in Danish 2nd Division was divided in two groups. The two winners were promoted to the 2008–09 Danish 1st Division, together with the winner of a promotion game between the two runners-up. Second squad teams can not promote, or play in the promotion game.
| Club | Group | Finishing position last season | First season of current spell in 2nd Divisions | |
|---|---|---|---|---|
| AaB 2 | West | 1st in Denmark Series Pool 2 | 2007-08 | |
| AB 70 | West | 12th | 2005–06 | |
| AGF 2 | West | 3rd in Denmark Series Pool 2 | 2007–08 | |
| B 1908 | East | 3rd in Denmark Series Pool 1 | 2007-08 | |
| B.93 | East | 6th | 2005–06 | |
| BK Skjold | East | 13th | 2006–07 | |
| BK Søllerød-Vedbæk | East | 13th | 2005–06 | |
| Brabrand IF | West | 14th in 1st Division | 2007–08 | |
| Brøndby 2 | East | 2nd | 2005–06 | |
| Brønshøj BK | East | 9th | 2006–07 | |
| Esbjerg 2 | West | 6th | 2005–06 | |
| FC Fyn | West | 3rd | 2004–05 | |
| FC Hjørring | West | 10th | 1999–00 | |
| FC Roskilde | East | 4th | 2004–05 | |
| Fremad Amager | East | 16th in 1st Division | 2007–08 | |
| Glostrup FK | East | 13th | 1999–00 | |
| Greve Fodbold | East | 10th | 2006–07 | |
| Hobro IK | West | 7th | 2006–07 | |
| Holbæk B&I | East | 8th | 2003–04 | |
| Holstebro BK | West | 12th | 1999–00 | |
| Ikast FS (FCM 2) | West | 2nd | 2006–07 | |
| Jetsmark IF | West | 5th | 2002–03 | |
| KB (FCK 2) | East | 5th | 2005–06 | |
| Næsby BK | West | 8th | 2004–05 | |
| OB 2 | West | 2nd in Denmark Series Pool 2 | 2007–08 | |
| Skovlunde IF | East | 11th | 2006–07 | |
| Slagelse B&I | East | 7th | 2002–03 | |
| Stenløse BK | East | 12th | 2005–06 | |
| Thisted FC | West | 15th in 1st Division | 2007–08 | |
| Vanløse IF | West | 1st in Denmark Series Pool 1 | 2007–08 | |
| Varde IF | West | 11th | 2005–06 | |
| Værløse BK | East | 1st in Denmark Series Pool 1 | 2007–08 |
The two runners-up will play promotion game on home and away basis.