The women's 400 metres hurdles event at the 2006 World Junior Championships in Athletics was held in Beijing, China, at Chaoyang Sports Centre on 15, 16 and 17 August.
| Gold | Kaliese Spencer | |
| Silver | Nicole Leach | |
| Bronze | Sherene Pinnock |
17 August
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 55.11 | ||||
| align=left | align=left | | 55.55 | ||||
| align=left | align=left | | 56.67 | ||||
| 4 | align=left | align=left | 57.03 | ||||
| 5 | align=left | align=left | 57.38 | ||||
| 6 | align=left | align=left |  | 57.47 | |||
| 7 | align=left | align=left |  | 58.49 | |||
| 8 | align=left | align=left | 59.37 |
16 August
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 56.10 | Q | ||
| 2 | align=left | align=left | | 57.11 | Q | ||
| 3 | align=left | align=left | | 57.19 | Q | ||
| 4 | align=left | align=left | | 57.42 | Q | ||
| 5 | align=left | align=left | 57.65 | ||||
| 6 | align=left | align=left |  | 57.69 | |||
| 7 | align=left | align=left |  | 58.05 | |||
| 8 | align=left | align=left |  | 58.55 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 56.11 | Q | ||
| 2 | align=left | align=left | 56.55 | Q | |||
| 3 | align=left | align=left | 57.00 | Q | |||
| 4 | align=left | align=left | 57.52 | Q | |||
| 5 | align=left | align=left |  | 57.65 | |||
| 6 | align=left | align=left | | 58.16 | |||
| 7 | align=left | align=left | 58.64 | ||||
| 8 | align=left | align=left | | 60.70 |
15 August
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.49 | Q | ||
| 2 | align=left | align=left | | 57.95 | Q | ||
| 3 | align=left | align=left | | 58.00 | Q | ||
| 4 | align=left | align=left | 58.04 | q | |||
| 5 | align=left | align=left |  | 59.22 | |||
| 6 | align=left | align=left |  | 60.10 | |||
| 7 | align=left | align=left | | 61.68 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.39 | Q | ||
| 2 | align=left | align=left | | 58.12 | Q | ||
| 3 | align=left | align=left | | 59.42 | Q | ||
| 4 | align=left | align=left |  | 59.48 | |||
| 5 | align=left | align=left | | 59.52 | |||
| 6 | align=left | align=left |  | 60.94 | |||
| 7 | align=left | align=left |  | 61.74 | |||
| 8 | align=left | align=left | 62.07 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.27 | Q | ||
| 2 | align=left | align=left | 58.60 | Q | |||
| 3 | align=left | align=left | | 58.88 | Q | ||
| 4 | align=left | align=left | 58.89 | q | |||
| 5 | align=left | align=left | | 59.58 | |||
| 6 | align=left | align=left | | 60.62 | |||
| 7 | align=left | align=left |  | 61.06 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | 57.11 | Q | |||
| 2 | align=left | align=left | | 57.35 | Q | ||
| 3 | align=left | align=left | 57.55 | Q | |||
| 4 | align=left | align=left | | 58.48 | q | ||
| 5 | align=left | align=left | | 59.14 | q | ||
| 6 | align=left | align=left | 61.30 | ||||
| 7 | align=left | align=left | 61.49 | ||||
| 8 | align=left | align=left |  | 63.54 |
According to an unofficial count, 30 athletes from 21 countries participated in the event.