| Year: | 2005 |
| Team: | Nicholls State Colonels |
| Sport: | football |
| Conference: | Southland Conference |
| Short Conf: | Southland |
| Tsnrank: | 17 |
| Record: | 6–4 |
| Conf Record: | 5–1 |
| Head Coach: | Jay Thomas |
| Hc Year: | 2nd |
| Off Coach: | H. T. Kinney |
| Oc Year: | 2nd |
| Stadium: | John L. Guidry Stadium |
| Champion: | Southland champion |
| Bowl: | NCAA Division I-AA First Round |
| Bowl Result: | L 12–14 at Furman |
The 2005 Nicholls State Colonels football team represented Nicholls State University as a member of the Southland Conference during the 2005 NCAA Division I-AA football season. Led by second-year head Jay Thomas, the Colonels compiled an overall record of 6–4 with a mark of 5–1 in conference play, sharing the Southland title with Texas State. Nicholls State advanced to the NCAA Division I-AA Football Championship playoffs, losing to Furman in the first round. The team played home games at John L. Guidry Stadium in Thibodaux, Louisiana.