The women's 400 metres hurdles event at the 2005 European Athletics U23 Championships was held in Erfurt, Germany, at Steigerwaldstadion on 15 and 16 July.
| Gold | Yelena Ildeykina | |
| Silver | Anastasiya Trifonova | |
| Bronze | Sian Scott |
16 July
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 56.43 | ||||
| align=left | align=left | | 56.51 | ||||
| align=left | align=left | 57.02 | |||||
| 4 | align=left | align=left | | 57.55 | |||
| 5 | align=left | align=left | 58.35 | ||||
| 6 | align=left | align=left |  | 58.66 | |||
| 7 | align=left | align=left |  | 58.69 | |||
| align=left | align=left |  | DQ | IAAF rule 168.7 |
15 July
Qualified: first 3 in each heat and 2 best to the Final
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.80 | Q | ||
| 2 | align=left | align=left | 58.17 | Q | |||
| 3 | align=left | align=left | | 58.47 | Q | ||
| 4 | align=left | align=left | 59.04 | q | |||
| 5 | align=left | align=left |  | 59.40 | |||
| 6 | align=left | align=left | | 59.94 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.57 | Q | ||
| 2 | align=left | align=left | | 58.30 | Q | ||
| 3 | align=left | align=left | | 58.59 | Q | ||
| 4 | align=left | align=left | | 58.64 | q | ||
| 5 | align=left | align=left | | 59.73 | |||
| 6 | align=left | align=left | 60.28 |
According to an unofficial count, 12 athletes from 8 countries participated in the event.
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