| Sport: | football |
| Year: | 2005 |
| Team: | Eastern Illinois Panthers |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Record: | 9–3 |
| Conf Record: | 8–0 |
| Head Coach: | Bob Spoo |
| Hc Year: | 19th |
| Def Coach: | Roc Bellantoni |
| Dc Year: | 4th |
| Stadium: | O'Brien Stadium |
| Champion: | OVC champion |
| Bowl: | NCAA Division I-AA First Round |
| Bowl Result: | L 6–21 vs. Southern Illinois |
The 2005 Eastern Illinois Panthers football team represented Eastern Illinois University as a member of the Ohio Valley Conference (OVC) during the 2005 NCAA Division I-AA football season. Led by 19th-year head coach Bob Spoo, the Panthers compiled an overall record of 9–3 with a mark of 8–0 in conference play, winning the OVC title. Eastern Illinois was invited to the NCAA Division I-AA Football Championship playoffs, where they lost Southern Illinois in the first round.