See main article: 2005 Copa Colsanitas Seguros Bolivar.
Four-time champion Fabiola Zuluaga failed to defend her title after losing to Lourdes Domínguez Lino in the semifinals. The loss also ends Zuluaga's 17-match winning streak at her home soil.
Flavia Pennetta won the title by defeating Lourdes Domínguez Lino 7–6(7–4), 6–4 in the final.