| Election Name: | Jacksonville mayoral election, 2003 |
| Country: | Jacksonville |
| Type: | presidential |
| Previous Election: | 1999 Jacksonville mayoral election |
| Previous Year: | 1999 |
| Next Election: | 2007 Jacksonville mayoral election |
| Next Year: | 2007 |
| Election Date: | May 13, 2003 |
| Nominee1: | John Peyton |
| Party1: | Nonpartisan candidate |
| Popular Vote1: | 110,778 |
| Percentage1: | 57.03% |
| Nominee2: | Nat Glover |
| Party2: | Nonpartisan candidate |
| Popular Vote2: | 83,480 |
| Percentage2: | 42.97% |
| Mayor | |
| Before Election: | John Delaney |
| Before Party: | Republican |
| After Election: | John Peyton |
| After Party: | Republican Party (United States) |
The Jacksonville mayoral election of 2003 took place on May 13, 2003. The two candidates to advance to the runoff were Republican millionaire John Peyton, and Democratic former Sheriff of Jacksonville Nat Glover. John Peyton won the election.