The women's 400 metres hurdles event at the 2003 European Athletics U23 Championships was held in Bydgoszcz, Poland, at Zawisza Stadion on 18 and 19 July.
| Gold | Oksana Gulumyan | |
| Silver | Irena Žauna | |
| Bronze | Maria Rus |
19 July
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 56.23 | ||||
| align=left | align=left | 56.47 | |||||
| align=left | align=left | | 57.01 | ||||
| 4 | align=left | align=left | | 57.37 | |||
| 5 | align=left | align=left |  | 57.61 | |||
| 6 | align=left | align=left |  | 58.06 | |||
| 7 | align=left | align=left |  | 58.15 | |||
| 8 | align=left | align=left | | 58.47 |
18 July
Qualified: first 2 in each heat and 2 best to the Final
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.50 | Q | ||
| 2 | align=left | align=left | | 57.83 | Q | ||
| 3 | align=left | align=left | | 57.84 | q | ||
| 4 | align=left | align=left |  | 59.34 | |||
| 5 | align=left | align=left |  | 59.81 | |||
| 6 | align=left | align=left |  | 61.02 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 58.18 | Q | ||
| 2 | align=left | align=left | | 58.58 | Q | ||
| 3 | align=left | align=left | 58.95 | ||||
| 4 | align=left | align=left | 59.93 | ||||
| 5 | align=left | align=left | | 60.26 | |||
| 6 | align=left | align=left |  | 88.69 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 55.98 | Q | ||
| 2 | align=left | align=left | 56.72 | Q | |||
| 3 | align=left | align=left | | 57.13 | q | ||
| 4 | align=left | align=left |  | 58.18 | |||
| 5 | align=left | align=left | 58.40 | ||||
| 6 | align=left | align=left |  | 59.67 | |||
| 7 | align=left | align=left |  | 61.30 |
According to an unofficial count, 19 athletes from 16 countries participated in the event.
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