| Champ: | Angelique Widjaja |
| Runner: |  Dinara Safina |
| Score: | 6–4, 0–6, 7–5 |
| Draw: | 64 (8 / 8) |
| Seeds: | 16 |
| Before Name: | Wimbledon Championships – Girls' singles |
| After Name: | Wimbledon Championships – Girls' singles |
See main article: 2001 Wimbledon Championships. María Emilia Salerni was the defending champion but did not complete in the Juniors this year.
Angelique Widjaja defeated Dinara Safina in the final, 6–4, 0–6, 7–5 to win the girls' singles tennis title at the 2001 Wimbledon Championships.[1]
See also: 1 and 1. 
Ashley Harkleroad (third round)
See also: 2 and 4. Jelena Janković (first round)
See also: 3 and 3. 
Claudine Schaul (third round)
See also: 4 and 2. Svetlana Kuznetsova (quarterfinals)
See also: 5 and 4. 
Eva Birnerová (third round)
See also: 6 and 2. 
Elena Baltacha (semifinals)
See also: 7 and 3. 
Gisela Dulko (third round)
See also: 8 and 1. Angelique Widjaja (champion)
See also: 9 and 4. Anne Keothavong (semifinals)
See also: 10 and 1. 
Marion Bartoli (second round)
See also: 11 and 2. Matea Mezak (third round)
See also: 12 and 3. Edina Gallovits (first round)
See also: 13 and 2. 
Sofia Arvidsson (third round)
See also: 14 and 1. Barbora Strýcová (quarterfinals)
See also: 15 and 3. Dinara Safina (final)
See also: 16 and 4. 
Monique Adamczak (second round)