| Country: | Florida |
| Flag Image: | Flag of St. Petersburg, Florida.svg |
| Type: | presidential |
| Ongoing: | no |
| Previous Election: | 1997 St. Petersburg, Florida, mayoral election |
| Previous Year: | 1997 |
| Next Election: | 2005 St. Petersburg, Florida, mayoral election |
| Next Year: | 2005 |
| Election Date: | February 27, 2001 (first-round) March 27, 2001 (runoff) |
| Turnout: | 25.59% (first round) 34.09% (runoff) |
| 1Blank: | First-round vote |
| 2Blank: | First-round percentage |
| 3Blank: | Second-round vote |
| 4Blank: | Second-round percentage |
| Candidate1: | Rick Baker |
| Party1: | nonpartisan candidate |
| 1Data1: | 9,306 |
| 2Data1: | 25.06% |
| 3Data1: | 27,841 |
| 4Data1: | 56.04% |
| Candidate2: | Kathleen S. Ford |
| Party2: | nonpartisan candidate |
| 1Data2: | 7,883 |
| 2Data2: | 21.23% |
| 3Data2: | 21,390 |
| 4Data2: | 43.06% |
| Candidate3: | Larry J. Williams |
| Party3: | nonpartisan candidate |
| 1Data3: | 7,664 |
| 2Data3: | 20.64% |
| Candidate4: | Karl Nurse |
| Party4: | nonpartisan candidate |
| 1Data4: | 5,498 |
| 2Data4: | 14.81% |
| Candidate5: | Omali Yeshitela |
| Party5: | nonpartisan candidate |
| 1Data5: | 3,905 |
| 2Data5: | 10.52% |
| Mayor | |
| Before Election: | David J. Fischer |
| After Election: | Rick Baker |
| After Party: | Republican Party (United States) |
St. Petersburg, Florida, held an election for mayor on February 27 and March 27, 2001. A non-partisan primary election was first held on February 27. No candidate won a majority of the vote, so the top two finisher advanced to a runoff.