| Year: | 2001 |
| Team: | Southern Miss Golden Eagles |
| Sport: | football |
| Conference: | Conference USA |
| Short Conf: | C-USA |
| Record: | 6–5 |
| Conf Record: | 4–3 |
| Head Coach: | Jeff Bower |
| Hc Year: | 12th |
| Off Coach: | Chris Klenakis |
| Oc Year: | 2nd |
| Off Scheme: | Multiple |
| Def Coach: | Tyrone Nix |
| Dc Year: | 1st |
| Def Scheme: | 3–4 |
| Stadium: | M. M. Roberts Stadium |
The 2001 Southern Miss Golden Eagles football team represented the University of Southern Mississippi in the 2001 NCAA Division I-A football season. The Golden Eagles were led by head coach Jeff Bower and played their home games at M. M. Roberts Stadium. They were a member of Conference USA.