| Gender: | Women's |
| Year: | 2001 |
| Division: | III |
| Teams: | 50 |
| Finalfourcity: | Danbury, Connecticut |
| Finalfourarena: | O'Neill Center |
| Champions: | Washington University Bears |
| Titlecount: | 4th |
| Runnerup: | Messiah Falcons |
| Gamecount: | 1st |
| Thirdplace: | Emmanuel Saints |
| Finalfourcount: | 1st |
| Fourthplace: | Ohio Wesleyan Battling Bishops |
| Finalfourcount2: | 1st |
| Coach: | Nancy Fahey |
| Coachcount: | 4th |
| Mop: | Tasha Rodgers |
| Mopteam: | Washington-St. Louis |
| Attendance: | 30212 |
The 2001 NCAA Division III women's basketball tournament was the 20th annual tournament hosted by the NCAA to determine the national champion of Division III women's collegiate basketball in the United States.[1]
Three-time defending champions Washington University in St. Louis defeated Messiah University in the championship game, 67–45, to claim the Bears' fourth Division III national title, their fourth of four consecutive.
The championship rounds were hosted at the O'Neill Center at Western Connecticut State University in Danbury, Connecticut from March 16–17, 2001.