| Competition: | Japan Football League |
| Season: | 2001 |
| Dates: | 1 April – 18 November |
| Winners: | Honda Motors 1st JFL title 2nd D3 title |
| Promoted: | none |
| League Topscorer: | Takaaki Tokushige (25 goals total) |
| Matches: | 240 |
| Total Goals: | 779 |
| Highest Attendance: | 3,305 (Round 28, Honda vs. Kyoken) |
| Lowest Attendance: | 48 (Round 23, Express vs. Sangyo) |
| Average Attendance: | 515 |
| Prevseason: | 2000 |
| Nextseason: | 2002 |
The was the third season of the Japan Football League, the third tier of the Japanese football league system.
It was contested by 16 teams, and Honda Motors won the championship. No promotion or relegation took place due to the promotion-eligible teams not being autonomous clubs fit for the J. League, and at the other end of the table, due to the expansion of the league from 16 to 18 teams.
| Rank | Scorer | Club | Goals |
|---|---|---|---|
| 1 |  Takaaki Tokushige | Denso SC | |
| 2 | Yasuhiko Niimura | Jatco SC | |
| 3 | Takehiro Hayashi | Otsuka Pharmaceuticals | |
| 4 | Mitsuru Hasegawa | YKK FC | |
| Masao Yamamoto | Sagawa Express | ||
No relegation has occurred due to expansion of the league to 18 teams. At the end of the season, the winner and runner-up of the Regional League promotion series, Sagawa Express Osaka and Profesor Miyazaki were promoted automatically.