| Year: | 2001 |
| Team: | Jacksonville State Gamecocks |
| Sport: | football |
| Conference: | Southland Football League |
| Short Conf: | Southland |
| Record: | 5–6 |
| Conf Record: | 2–4 |
| Head Coach: | Jack Crowe |
| Hc Year: | 2nd |
| Off Coach: | Willie J. Slater |
| Oc Year: | 2nd |
| Def Coach: | Greg Stewart |
| Dc Year: | 2nd |
| Stadium: | Paul Snow Stadium |
The 2001 Jacksonville State Gamecocks football team represented Jacksonville State University as a member of the Southland Football League during the 2001 NCAA Division I-AA football season. The Gamecocks compiled an overall record of 5–6 with a mark of 2–4 in conference play, placing fifth in the Southland. Jacksonville State played home games at Paul Snow Stadium in Jacksonville, Alabama.