The women's 4 x 400 metres relay event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 15 July.
| Gold | Karen Gear Jennifer Meadows Tracey Duncan Helen Thieme | |
| Silver | Aleksandra Pielużek Aneta Lemiesz Monika Bejnar Justyna Karolkiewicz  | |
| Bronze | Natalya Zhuravlyova Natalya Sidorenko Yuliya Gurtovenko Antonina Yefremova  |
15 July
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| align=left | 3:31.74 | |||||
| align=left | | 3:32.38 | ||||
| align=left | | 3:34.16 | ||||
| 4 | align=left |  | 3:35.08 | |||
| 5 | align=left |  | 3:37.01 | |||
| 6 | align=left | 3:37.33 |
According to an unofficial count, 24 athletes from 6 countries participated in the event.
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