The women's 4 x 100 metres relay event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 15 July.
| Gold | Susan Burnside Helen Roscoe Sabrina Scott Abiodun Oyepitan | |
| Silver | Yuliya Bartsevich Oksana Dragun Yelena Nevmerzhitskaya Yevgeniya Likhuta  | |
| Bronze | Elina Lax Heidi Hannula Johanna Manninen Katja Salivaara  |
15 July
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| align=left | 44.31 | |||||
| align=left | | 44.64 | ||||
| align=left | | 44.76 | ||||
| 4 | align=left |  | 44.80 | |||
| 5 | align=left | 45.11 | ||||
| align=left | DQ |
According to an unofficial count, 24 athletes from 6 countries participated in the event.
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