The women's 400 metres hurdles event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 13 and 14 July.
| Gold | Sylvanie Morandais | |
| Silver | Aleksandra Pielużek | |
| Bronze | Irēna Žauna |
14 July
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 56.30 | ||||
| align=left | align=left | | 56.51 | ||||
| align=left | align=left | 57.03 | |||||
| 4 | align=left | align=left | 57.26 | ||||
| 5 | align=left | align=left |  | 58.28 | |||
| 6 | align=left | align=left |  | 58.49 | |||
| 7 | align=left | align=left | | 58.95 | |||
| 8 | align=left | align=left |  | 59.75 |
13 July
Qualified: first 3 in each heat and 2 best to the Final
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.16 | Q | ||
| 2 | align=left | align=left | 57.23 | Q | |||
| 3 | align=left | align=left | | 58.32 | Q | ||
| 4 | align=left | align=left | | 58.34 | q | ||
| 5 | align=left | align=left | | 58.78 | q | ||
| 6 | align=left | align=left | 59.24 | ||||
| align=left | align=left | DNF |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | 57.66 | Q | |||
| 2 | align=left | align=left | | 58.56 | Q | ||
| 3 | align=left | align=left | | 59.07 | Q | ||
| 4 | align=left | align=left |  | 59.38 | |||
| 5 | align=left | align=left |  | 59.51 | |||
| 6 | align=left | align=left | | 59.90 | |||
| 7 | align=left | align=left | | 60.12 |
According to an unofficial count, 14 athletes from 11 countries participated in the event.
(1) (3) (1) (1) (2) (1) (1)