The women's 400 metres event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 12 and 13 July.
| Gold | Antonina Yefremova | |
| Silver | Helen Thieme | |
| Bronze | Aneta Lemiesz |
13 July
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 52.29 | ||||
| align=left | align=left | 52.75 | |||||
| align=left | align=left | | 53.25 | ||||
| 4 | align=left | align=left | | 53.26 | |||
| 5 | align=left | align=left | 53.91 | ||||
| 6 | align=left | align=left | 54.05 | ||||
| 7 | align=left | align=left |  | 54.31 | |||
| align=left | align=left |  | DNF |
12 July
Qualified: first 3 in each heat and 2 best to the Final
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 52.77 | Q | ||
| 2 | align=left | align=left | | 53.03 | Q | ||
| 3 | align=left | align=left | 53.31 | Q | |||
| 4 | align=left | align=left | 53.32 | q | |||
| 5 | align=left | align=left | | 53.76 | q | ||
| 6 | align=left | align=left |  | 54.92 | |||
| 7 | align=left | align=left |  | 55.53 | |||
| 8 | align=left | align=left | | 56.04 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 52.43 | Q | ||
| 2 | align=left | align=left | 52.88 | Q | |||
| 3 | align=left | align=left | | 53.47 | Q | ||
| 4 | align=left | align=left |  | 53.96 | |||
| 5 | align=left | align=left |  | 54.01 | |||
| 6 | align=left | align=left | 55.17 | ||||
| 7 | align=left | align=left |  | 55.23 |
According to an unofficial count, 15 athletes from 11 countries participated in the event.
(1) (1) (1) (1) (1) (1) (1) (1) (3)