The women's 100 metres hurdles event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 14 and 15 July.
| Gold | Susanna Kallur | |
| Silver | Jenny Kallur | |
| Bronze | Tessy Prediger |
15 July
Wind: 1.2 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 12.96 | ||||
| align=left | align=left | | 13.19 | ||||
| align=left | align=left | 13.31 | |||||
| 4 | align=left | align=left | 13.45 | ||||
| 5 | align=left | align=left |  | 13.46 | |||
| 6 | align=left | align=left |  | 13.48 | |||
| 7 | align=left | align=left |  | 13.57 | |||
| 8 | align=left | align=left |  | 13.90 |
14 July
Qualified: first 2 in each heat and 2 best to the Final
Wind: -0.3 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 13.45 | Q | ||
| 2 | align=left | align=left | | 13.58 | Q | ||
| 3 | align=left | align=left | | 13.67 | q | ||
| 4 | align=left | align=left | 13.72 | ||||
| 5 | align=left | align=left |  | 13.93 | |||
| 6 | align=left | align=left |  | 14.22 |
Wind: -1.2 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | 13.52 | Q | |||
| 2 | align=left | align=left | | 13.71 | Q | ||
| 3 | align=left | align=left | 13.75 | ||||
| 4 | align=left | align=left |  | 13.79 | |||
| 5 | align=left | align=left |  | 13.94 | |||
| 6 | align=left | align=left | | 19.68 |
Wind: -0.3 m/s
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 12.95 | Q, CR | ||
| 2 | align=left | align=left | 13.44 | Q | |||
| 3 | align=left | align=left | | 13.65 | q | ||
| 4 | align=left | align=left | | 13.78 | |||
| 5 | align=left | align=left |  | 14.30 |
According to an unofficial count, 17 athletes from 13 countries participated in the event.
(1) (1) (1) (3) (1) (1) (1) (1) (2) (1)