The men's 4 x 400 metres relay event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 14 and 15 July.
| Gold | David Naismith Adam Potter Richard McDonald Matthew Elias | |
| Silver | Simon Kirch Stefan Holz Ruwen Faller Marc Alexander Scheer | |
| Bronze | Oleg Mishukov Maksim Babarykin Dmitriy Bogdanov Yevgeniy Lebedev  |
15 July
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| align=left | 3:05.24 | |||||
| align=left | 3:05.39 | |||||
| align=left | | 3:06.41 | ||||
| 4 | align=left |  | 3:06.96 | |||
| 5 | align=left |  | 3:08.01 | |||
| 6 | align=left | 3:08.30 | ||||
| 7 | align=left |  | 3:08.39 | |||
| 8 | align=left |  | 3:14.24 |
14 July
Qualified: first 3 in each heat and 2 best to the Final
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| 1 | align=left | 3:07.33 | Q | |||
| 2 | align=left | | 3:07.39 | Q | ||
| 3 | align=left | 3:07.72 | Q | |||
| 4 | align=left | | 3:08.98 | q | ||
| 5 | align=left | 3:09.15 | q |
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| 1 | align=left | | 3:09.40 | Q | ||
| 2 | align=left | | 3:10.16 | Q | ||
| 3 | align=left | | 3:10.74 | Q | ||
| 4 | align=left |  | 3:10.77 | |||
| 5 | align=left | 3:11.10 |
According to an unofficial count, 40 athletes from 10 countries participated in the event.
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