The men's 4 x 100 metres relay event at the 2001 European Athletics U23 Championships was held in Amsterdam, Netherlands, at Olympisch Stadion on 14 and 15 July.
| Gold | Tomasz Kondratowicz Łukasz Chyła Marcin Płacheta Przemysław Rogowski  | |
| Silver | Jonathan O'Parka Jonathan Barbour Darren Chin Ben Lewis | |
| Bronze | Matic Šušteršic Matic Osovnikar Boštjan Fridrih Rok Orel |
15 July
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| align=left | | 39.41 | ||||
| align=left | 39.45 | |||||
| align=left | 39.95 | |||||
| 4 | align=left |  | 40.02 | |||
| 5 | align=left |  | 40.22 | |||
| align=left |  | DNF | ||||
| align=left | DNF | |||||
| align=left | DQ |
14 July
Qualified: first 3 in each heat and 2 best to the Final
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| 1 | align=left | | 39.73 | Q | ||
| 2 | align=left | 39.78 | Q | |||
| 3 | align=left | | 39.99 | Q | ||
| 4 | align=left | | 40.07 | q | ||
| 5 | align=left | 40.92 | q |
| Rank | Nation | Competitors | Time | Notes | ||
|---|---|---|---|---|---|---|
| 1 | align=left | 39.90 | Q | |||
| 2 | align=left | 40.18 | Q | |||
| 3 | align=left | | 41.05 | Q | ||
| align=left |  | DQ |
According to an unofficial count, 40 athletes from 9 countries participated in the event.
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