| Year: | 2001 |
| Team: | Eastern Illinois Panthers |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Tsnrank: | 9 |
| Record: | 9–2 |
| Conf Record: | 6–0 |
| Head Coach: | Bob Spoo |
| Hc Year: | 15th |
| Off Coach: | Roy Wittke |
| Oc Year: | 12th |
| Stadium: | O'Brien Stadium |
| Champion: | OVC champion |
| Bowl: | NCAA Division I-AA First Round |
| Bowl Result: | L 43–49 at Northern Iowa |
The 2001 Eastern Illinois Panthers represented Eastern Illinois University as a member of the Ohio Valley Conference (OVC) during the 2001 NCAA Division I-AA football season. Led by 15th-year head coach Bob Spoo, the Panthers compiled an overall record of 9–2, winning the OVC title with a conference mark of 6–1. Eastern Illinois was invited to the NCAA Division I-AA Football Championship playoffs, where they lost in the first round to Northern Iowa. The Bobcats were ranked ninth in the final Sports Network poll.