| 2001 CIS University Cup | |
| Venues: | Waterloo Recreation Complex, Waterloo, Ontario |
| Winners: | Quebec–Trois-Rivières Patriotes |
| Count: | 3 |
| Second: | St. Francis Xavier X-Men |
| Games: | 7 |
| Num Teams: | 6 |
| Award Title: | MVP |
| Prev Season: | 2000 |
| Next Season: | 2002 |
The 2001 CIS Men's University Cup Hockey Tournament (39th annual) was held at the Waterloo Recreation Complex in Waterloo, Ontario. The Wilfrid Laurier Golden Hawks served as primary tournament host with assistance from the Guelph Gryphons and Waterloo Warriors.
Note: * denotes overtime period(s)
Note: * denotes overtime period(s)
Note: * denotes overtime period(s)
The rotating wild-card moved to AUS. In order to prevent both AUS teams from being in the same group, Western Ontario and St. Thomas switched places. The teams were then seeded accordingly.
| Seed | Team | Qualification | Record | Appearance | Last | |
|---|---|---|---|---|---|---|
| 1 | West: Canada West Champion | 29–2–2 | 26th | 2000 | ||
| 2 | Quebec: OUA Champion | 25–1–2 | 11th | 2000 | ||
| 3 | Atlantic: AUS Champion | 20–11–4 | 1st | Never | ||
| 4 | Ontario: OUA Runner-up | 24–4–0 | 6th | 2000 | ||
| 5 | Wild-card: AUS Runner-up | 23–5–5 | 7th | 1978 | ||
| 6 | Host | 10–13–4 | 6th | 1992 |
Note: * denotes overtime period(s)[1]