| Champ: |  Marat Safin |
| Runner: |  Harel Levy |
| Score: | 6–2, 6–3 |
See main article: 2000 du Maurier Open.
Marat Safin defeated Harel Levy in the final, 6–2, 6–3 to win the men's singles tennis title at the 2000 Canadian Open. Levy became the first Israeli to reach a Master 1000 single final.
Thomas Johansson was the defending champion, but lost in the second round to Wayne Ferreira.
Andre Agassi (first round) Pete Sampras (quarterfinals)
Magnus Norman (first round)
Gustavo Kuerten (second round) Yevgeny Kafelnikov (quarterfinals) Thomas Enqvist (third round, retired)
Lleyton Hewitt (second round) Marat Safin (champion)
Nicolás Lapentti (first round) Juan Carlos Ferrero (third round)
Franco Squillari (first round) Patrick Rafter (quarterfinals)
Tim Henman (first round) Mark Philippoussis (first round)
Nicolas Escudé (third round, retired)