| Sport: | football |
| Year: | 2000 |
| Team: | Wyoming Cowboys |
| Conference: | Mountain West Conference |
| Short Conf: | MW |
| Record: | 1–10 |
| Conf Record: | 0–7 |
| Hc Year: | 1st |
| Off Coach: | Rusty Burns |
| Oc Year: | 1st |
| Def Coach: | Matt Wallerstedt |
| Dc Year: | 1st as DC, 4th overall |
The 2000 Wyoming Cowboys football team represented the University of Wyoming in the 2000 NCAA Division I-A football season. The Cowboys offense scored 170 points, while the defense allowed 393 points.
The following were selected in the 2001 NFL draft.
| Player | Position | Round | Overall | NFL team |
| Patrick Chukwurah | Linebacker | 5 | 157 | Minnesota Vikings |