The women's pole vault event at the 2000 World Junior Championships in Athletics was held in Santiago, Chile, at Estadio Nacional Julio Martínez Prádanos on 18 October.
| Gold | Yelena Isinbaeva | |
| Silver | Annika Becker | |
| Bronze | Vanessa Boslak | |
| Bronze | Fanni Juhász |
18 October
| Rank | Name | Nationality | Result | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 4.20 | ||||
| align=left | align=left | 4.10 | |||||
| align=left | align=left | | 4.10 | ||||
| align=left | align=left | | 4.10 | ||||
| 5 | align=left | align=left | 4.00 | ||||
| 6 | align=left | align=left |  | 3.70 | |||
| 6 | align=left | align=left |  | 3.70 | |||
| 6 | align=left | align=left | | 3.70 | |||
| 9 | align=left | align=left |  | 3.70 | |||
| 10 | align=left | align=left |  | 3.70 | |||
| 11 | align=left | align=left | | 3.50 | |||
| 11 | align=left | align=left |  | 3.50 | |||
| 13 | align=left | align=left |  | 3.50 | |||
| 14 | align=left | align=left | 3.50 | ||||
| 14 | align=left | align=left | 3.50 | ||||
| align=left | align=left |  | NH | ||||
| align=left | align=left |  | NH | ||||
| align=left | align=left |  | NH | ||||
| align=left | align=left | | NH |
According to an unofficial count, 19 athletes from 15 countries participated in the event.