The women's 800 metres event at the 2000 World Junior Championships in Athletics was held in Santiago, Chile, at Estadio Nacional Julio Martínez Prádanos on 17, 18 and 20 October.
| Gold | Nancy Jebet Langat | |
| Silver | Georgie Clarke | |
| Bronze | Lucia Klocová |
20 October
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 2:01.51 | ||||
| align=left | align=left | | 2:02.28 | ||||
| align=left | align=left | | 2:04.00 | ||||
| 4 | align=left | align=left |  | 2:04.60 | |||
| 5 | align=left | align=left |  | 2:06.55 | |||
| 6 | align=left | align=left |  | 2:07.26 | |||
| 7 | align=left | align=left | | 2:08.97 | |||
| align=left | align=left | DNS |
18 October
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 2:06.77 | Q | ||
| 2 | align=left | align=left | | 2:06.82 | Q | ||
| 3 | align=left | align=left | | 2:07.29 | Q | ||
| 4 | align=left | align=left | 2:07.31 | q | |||
| 5 | align=left | align=left |  | 2:08.34 | |||
| 6 | align=left | align=left |  | 2:08.62 | |||
| 7 | align=left | align=left | | 2:11.96 | |||
| 8 | align=left | align=left | | 2:14.40 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 2:06.53 | Q | ||
| 2 | align=left | align=left | | 2:06.67 | Q | ||
| 3 | align=left | align=left | | 2:06.83 | Q | ||
| 4 | align=left | align=left | | 2:07.35 | q | ||
| 5 | align=left | align=left |  | 2:07.46 | |||
| 6 | align=left | align=left | | 2:09.67 | |||
| 7 | align=left | align=left | 2:10.09 | ||||
| 8 | align=left | align=left |  | 2:10.51 |
17 October
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 2:06.48 | Q | ||
| 2 | align=left | align=left | | 2:07.86 | Q | ||
| 3 | align=left | align=left | | 2:08.81 | Q | ||
| 4 | align=left | align=left | | 2:09.47 | q | ||
| 5 | align=left | align=left | 2:11.17 | ||||
| align=left | align=left | DNF |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 2:07.79 | Q | ||
| 2 | align=left | align=left | 2:07.82 | Q | |||
| 3 | align=left | align=left | | 2:08.41 | Q | ||
| 4 | align=left | align=left | | 2:09.39 | q | ||
| 5 | align=left | align=left | | 2:09.50 | q | ||
| 6 | align=left | align=left | 2:16.54 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 2:08.35 | Q | ||
| 2 | align=left | align=left | | 2:08.78 | Q | ||
| 3 | align=left | align=left | | 2:08.79 | Q | ||
| 4 | align=left | align=left | 2:09.77 | q | |||
| 5 | align=left | align=left |  | 2:11.23 | |||
| 6 | align=left | align=left | 2:20.17 | ||||
| align=left | align=left | DQ |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 2:10.11 | Q | ||
| 2 | align=left | align=left | | 2:10.20 | Q | ||
| 3 | align=left | align=left | | 2:10.41 | Q | ||
| 4 | align=left | align=left | 2:10.56 | ||||
| 5 | align=left | align=left |  | 2:10.78 | |||
| 6 | align=left | align=left |  | 2:11.78 |
According to an unofficial count, 25 athletes from 20 countries participated in the event.