| Champ: |  María Emilia Salerni |
| Runner: |  Tatiana Perebiynis |
| Score: | 6–4, 7–5 |
| Draw: | 64 (8 / 8) |
| Seeds: | 16 |
| Before Name: | Wimbledon Championships – Girls' singles |
| After Name: | Wimbledon Championships – Girls' singles |
See main article: 2000 Wimbledon Championships. The 2000 Junior Championships, Wimbledon 2000 Tournament, took place between June 29 and July 9, 2000 in Great Britain. María Emilia Salerni defeated Tatiana Perebiynis in the final, 6–4, 7–5 to win the girls' singles tennis title at the 2000 Wimbledon Championships.[1] Iroda Tulyaganova was the defending champion but did not complete in the Juniors this year.
See also: 1 and 1. 
Dája Bedáňová (first round)
See also: 2 and 4. Tatiana Perebiynis (final)
See also: 3 and 3. 
Marie-Gaïané Mikaelian (semifinals)
See also: 4 and 2. María Emilia Salerni (champion)
See also: 5 and 2. 
Anikó Kapros (quarterfinals)
See also: 6 and 3. 
Christina Wheeler (quarterfinals)
See also: 7 and 1. Ioana Gaspar (semifinals)
See also: 8 and 4. 
Hannah Collin (third round)
See also: 9 and 1. 
Lenka Dlhopolcová (third round)
See also: 10 and 3. Gisela Dulko (first round)
See also: 11 and 3. Renata Voráčová (third round)
See also: 12 and 2. Eva Birnerová (first round)
See also: 13 and 2. 
Alyssa Cohen (third round)
See also: 14 and 4. Ľubomíra Kurhajcová (second round)
See also: 15 and 1. Yuliya Beygelzimer (second round)
See also: 16 and 4. 
Claudine Schaul (quarterfinals)