| Champ: |  Nicolas Mahut |
| Runner: | Mario Ančić |
| Score: | 3–6, 6–3, 7–5 |
| Draw: | 64 (8 / 8) |
| Seeds: | 16 |
| Before Name: | Wimbledon Championships – Boys' singles |
| After Name: | Wimbledon Championships – Boys' singles |
See main article: 2000 Wimbledon Championships. Jürgen Melzer was the defending champion, but did not complete in the Juniors this year.
Nicolas Mahut defeated Mario Ančić in the final, 3–6, 6–3, 7–5 to win the boys' singles tennis title at the 2000 Wimbledon Championships.[1]
See also: 1 and 1. Nicolas Mahut (champion)
See also: 2 and 4. Todor Enev (semifinals)
See also: 3 and 2. 
Joachim Johansson (second round)
See also: 4 and 3. Mario Ančić (final)
See also: 5 and 4. Andrej Kračman (quarterfinals)
See also: 6 and 3. Adrian Cruciat (first round)
See also: 7 and 1. Maximilian Abel (first round)
See also: 8 and 2. 
Andriy Dernovskiy (second round)
See also: 9 and 1. Roman Valent (third round)
See also: 10 and 4. Lu Yen-hsun (first round)
See also: 11 and 2. 
Alejandro Falla (second round)
See also: 12 and 3. 
Michal Kokta (third round)
See also: 13 and 1. Janko Tipsarević (first round)
See also: 14 and 2. 
Bruno Soares (first round)
See also: 15 and 4. 
Cristian Villagrán (third round)
See also: 16 and 3. 
Kristof Vliegen (quarterfinals)