| Election Name: | 2000 United States Senate election in Wisconsin |
| Country: | Wisconsin |
| Type: | presidential |
| Ongoing: | no |
| Previous Election: | 1994 United States Senate election in Wisconsin |
| Previous Year: | 1994 |
| Next Election: | 2006 United States Senate election in Wisconsin |
| Next Year: | 2006 |
| Election Date: | November 7, 2000 |
| Image1: | File:Herbert Kohl, official photo.jpg |
| Nominee1: | Herb Kohl |
| Party1: | Democratic Party (United States) |
| Popular Vote1: | 1,563,238 |
| Percentage1: | 61.54% |
| Nominee2: | John Gillespie |
| Party2: | Republican Party (United States) |
| Popular Vote2: | 940,744 |
| Percentage2: | 37.04% |
| U.S. Senator | |
| Before Election: | Herb Kohl |
| Before Party: | Democratic Party (United States) |
| After Election: | Herb Kohl |
| After Party: | Democratic Party (United States) |
The 2000 United States Senate election in Wisconsin took place on November 7, 2000. Incumbent Democratic U.S. Senator Herb Kohl won re-election to a third term by a margin of 24.5%. Herb Kohl defeated John Gillespie in a landslide despite Al Gore narrowly winning Wisconsin over Republican presidential nominee George Walker Bush in the concurrent presidential election.