See main article: 2000 US Open (tennis).
Venus Williams defeated Lindsay Davenport in the final, 6–4, 7–5 to win the women's singles tennis title at the 2000 US Open. It was her second major singles title, and she became the second woman to win Wimbledon, the Olympics, and the US Open in the same season (after Steffi Graf in 1988).
Serena Williams was the defending champion, but was defeated in the quarterfinals by Davenport in a rematch of the previous year's semifinal.
This was the first major appearance for future French Open champion Francesca Schiavone. She entered the main draw as a qualifier and lost to Jelena Dokić in the third round.
See main article: article and 2000 US Open – Women's singles qualifying.