See main article: 2000 US Open (tennis).
Marat Safin defeated Pete Sampras in the final, 6–4, 6–3, 6–3 to win the men's singles tennis title at the 2000 US Open. It was his first major title.
Andre Agassi was the defending champion, but lost in the second round to Arnaud Clément.
The tournament marked the first major main draw appearance of future world No. 1 and 2003 US Open champion Andy Roddick, and the first US Open main draw appearance of future world No. 1 and five-time US Open champion Roger Federer.
The seeded players are listed below. Marat Safin is the champion; others show the round in which they were eliminated.
Andre Agassi (second round)
Gustavo Kuerten (first round)
Magnus Norman (fourth round) Pete Sampras (finalist)
Yevgeny Kafelnikov (third round) Marat Safin (champion) Thomas Enqvist (fourth round)
Àlex Corretja (third round)
Lleyton Hewitt (semifinalist)
Cédric Pioline (third round)
Tim Henman (third round) Juan Carlos Ferrero (fourth round)
Franco Squillari (second round)
Nicolas Kiefer (quarterfinalist) Mark Philippoussis (second round)
Nicolás Lapentti (second round)See main article: 2000 US Open – Men's singles qualifying.