| Year: | 2000 |
| Team: | Tuskegee Golden Tigers |
| Sport: | football |
| Conference: | Southern Intercollegiate Athletic Conference |
| Short Conf: | SIAC |
| Record: | 12–0 |
| Conf Record: | 7–0 |
| Head Coach: | Rick Comegy |
| Hc Year: | 5th |
| Stadium: | Abbott Memorial Alumni Stadium |
| Champion: | Black college national champion SIAC champion |
| Bowl: | Pioneer Bowl |
| Bowl Result: | W 12–9 vs. |
The 2000 Tuskegee Golden Tigers football team represented Tuskegee University as a member of the Southern Intercollegiate Athletic Conference (SIAC) during the 2000 NCAA Division II football season. Led by fifth-year head coach Rick Comegy, the Golden Tigers compiled an overall record of 12–0, with a conference record of 7–0, and finished as SIAC champion. At the conclusion of the season, the Golden Tigers were also recognized as black college national champion.