| Year: | 2000 |
| Team: | Tennessee State Tigers |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Record: | 3–8 |
| Conf Record: | 2–5 |
| Head Coach: | James Reese |
| Hc Year: | 1st |
| Stadium: | Adelphia Coliseum |
The 2000 Tennessee State Tigers football team represented Tennessee State University as a member of the Ohio Valley Conference (OVC) during the 2000 NCAA Division I-AA football season. Led by first-year head coach James Reese, the Tigers compiled an overall record of 3–8, with a conference record of 2–6, and finished sixth in the OVC.