| Flag Image: | Flag of San Juan, Puerto Rico.svg |
| Country: | Puerto Rico |
| Type: | presidential |
| Previous Election: | 1996 San Juan, Puerto Rico, mayoral election |
| Previous Year: | 1996 |
| Next Election: | 2004 San Juan, Puerto Rico, mayoral election |
| Next Year: | 2004 |
| Election Date: | November 7, 2000 |
| Image1: | Jorge Santini Padilla, Mayor of San Juan, Puerto Rico (cropped) (a).jpg |
| Nominee1: | Jorge Santini |
| Party1: | New Progressive Party (Puerto Rico) |
| Popular Vote1: | 101,012 |
| Percentage1: | 47.98% |
| Nominee2: | Eduardo Bhatia |
| Party2: | Popular Democratic Party (Puerto Rico) |
| Alliance2: | Democratic |
| Popular Vote2: | 97,383 |
| Percentage2: | 46.26% |
| Nominee3: | Vance Thomas |
| Party3: | Puerto Rican Independence Party |
| Popular Vote3: | 10,753 |
| Percentage3: | 5.11% |
| Mayor | |
| Before Election: | Sila María Calderón |
| Before Party: | Popular Democratic Party (Puerto Rico) |
| After Election: | Jorge Santini |
| After Party: | New Progressive Party (Puerto Rico) |
| Turnout: | 79.34% |
San Juan, Puerto Rico, held an election for mayor on November 7, 2000. It was held as part of the 2000 Puerto Rican general election. It saw the election of Jorge Santini, a member of the New Progressive Party.
Incumbent mayor Sila María Calderón, a member of the Popular Democratic Party, did not seek reelection to a second term, and instead ran for governor.