| Competition: | Russian Second Division |
| Season: | 2000 |
| Prevseason: | 1999 |
| Nextseason: | 2001 |
The 2000 Russian Second Division was the ninth edition of the Russian Second Division. There were 6 zones with 107 teams starting the competition (one was excluded before the end of the season).
| Team | Head coach |
|---|---|
| FC Severstal Cherepovets | Boris Rappoport |
| FC Dynamo Vologda | Vladimir Yulygin |
| FC Pskov |  Eduard Malofeyev (until August)Aleksandr Lebedev (from August) |
| FC Spartak Shchyolkovo | Aleksandr Ignatenko (until June) Yevgeni Sidorov (from June) |
| FC Mosenergo Moscow | Valentin Sysoyev |
| FC Torpedo-2 Moscow | Sergei Petrenko |
| FC Volochanin-89 Vyshny Volochyok | Viktor Demidov |
| FC Neftyanik Yaroslavl | |
| FC Zenit-2 St. Petersburg | |
| FC Avtomobilist Noginsk | Igor Volchok |
| FC Sportakademklub Moscow | Sergei Abramov |
| FC Lokomotiv-2 Moscow | Andrei Syomin |
| PFC CSKA-2 Moscow | |
| FC Oazis Yartsevo | Leonid Arkhipov (until August) Ivan Burlaku (from August) |
| FC Spartak-2 Moscow | Nikolay Kiselyov (until May) |
| FC Spartak Kostroma | Nikolai Semykin (until May) Aleksei Yarovoy (June) Viktor Nozdrin (from July) |
| FC Dynamo-2 Moscow | Leonid Ablizin |
| FC Saturn-d Ramenskoye | |
| FC Energiya Velikiye Luki | Vladimir Kosogov (until July) Vladimir Tsvetkov (from July) |
| Team | Head coach |
|---|---|
| FC Kuban Krasnodar | Soferbi Yeshugov |
| FC SKA Rostov-on-Don | Aleksandr Tumasyan |
| FC Kavkazkabel Prokhladny | Sergei Razaryonov |
| FC Avtodor Vladikavkaz | Yuri Gazzaev |
| FC Druzhba Maykop | Adam Natkho |
| FC Angusht Nazran | Timur Kuriyev (until July) Umar Markhiyev (from August) |
| FC Vityaz Krymsk | Kalin Stepanyan |
| FC Lokomotiv-Taym Mineralnye Vody | Aleksandr Ikayev (until April) Yuri Dukhovnov (April to August) Aleksandr Babayan (from August) |
| FC Rostselmash-2 Rostov-on-Don | |
| FC Dynamo Makhachkala | Magomed Isayev |
| FC Mozdok | Vladimir Arkalov |
| FC Slavyansk Slavyansk-na-Kubani | Vladimir Sinyagovsky |
| FC Shakhtyor Shakhty | Valeri Dzolov |
| FC Zhemchuzhina-2 Sochi | Anatoli Lyz |
| FC Venets Gulkevichi | Vasili Sidorenko |
| FC Nart Nartkala | Vladimir Kurashinov |
| FC Iriston Vladikavkaz | Ruslan Merdenov |
| FC Torpedo Taganrog | Anatoli Shabanov |
| FC Beshtau Lermontov | Sergei Shestakov, Viktor Vashchenko |
| FC Torpedo Georgiyevsk | Aleksandr Ivanchenko |
| Team | Head coach |
|---|---|
| FC Svetotekhnika Saransk | Aleksandr Khomutetskiy |
| FC Torpedo Pavlovo | Aleksandr Sarafannikov |
| FC Volga Ulyanovsk | Sergei Sedyshev |
| FC Metallurg Vyksa | Yevgeni Popov |
| FC Balakovo | Gennadi Drozdov |
| FC Torpedo-Viktoriya Nizhny Novgorod | Anatoli Nefyodov |
| FC Energetik Uren | Viktor Pavlyukov |
| FC Iskra Engels | Yuri Vasilyev |
| FC Lada-Energiya Dimitrovgrad | Andrei Flaat |
| FC Olimpiya Volgograd | |
| FC Diana Volzhsk | Valeri Bogdanov |
| FC Biokhimik-Mordovia Saransk | Igor Shinkarenko |
| FC Khimik Dzerzhinsk | Valeri Volodin |
| FC Rotor-2 Volgograd | |
| FC Khopyor Balashov | |
| FC Torpedo Volzhsky | Oleg Dudarin, Anatoli Kurbatov, Valeri Chupin, Viktor Ionov |
| FC Krylya Sovetov-2 Samara | |
| FC Salyut Saratov | Vladimir Khoroltsev |
| Team | Head coach |
|---|---|
| FC Neftekhimik Nizhnekamsk | Ivan Butaliy |
| FC Uralmash Yekaterinburg | Nikolai Agafonov |
| FC KAMAZ-Chally Naberezhnye Chelny | Pyotr Shubin |
| FC Tyumen | Viktor Knyazhev (until July) Rudolf Atamalyan (from July) |
| FC Sodovik Sterlitamak | Sergei Maksimov |
| FC Metallurg-Metiznik Magnitogorsk | Aleksandr Kukushkin |
| FC Zenit Chelyabinsk | Oleg Kudelin, Rafail Fazylov |
| FC Uralets Nizhny Tagil | Lev Kutashov |
| FC Gazovik Orenburg | Aleksandr Korolyov |
| FC UralAZ Miass | Pyotr Nagayev, Valeri Znarok |
| FC Dynamo Perm | Andrei Boglayevskiy |
| FC Energiya Chaikovsky | Sergei Kleymyonov |
| FC Dynamo-Mashinostroitel Kirov | Vasili Esaulov, Aleksandr Kishinevsky |
| FC Dynamo Izhevsk | Stanislav Korotayev |
| FC Spartak Yoshkar-Ola | Aleksandr Yerastov, Viktor Zvyagin, Igor Pochuyenkov, Aleksandr Rasputin |
| FC Spartak Kurgan | Ivan Gerasimov |
| Team | Head coach |
|---|---|
| FC Metallurg Novokuznetsk | Vladislav Sosnov (until August) Sergei Synovyat (from August) |
| FC SKA-Energiya Khabarovsk |  Oleh Smolyaninov |
| FC Zvezda Irkutsk | Sergei Muratov, Gennadi Vyazovtsev |
| FC Luch Vladivostok | Yuri Karamyan (until August) Boris Zhuravlyov (from August) |
| FC Okean Nakhodka | Vladimir Susin |
| FC Chkalovets-Olimpik Novosibirsk | Yuri Gavrilov |
| FC Irtysh Omsk | Sergei Vasyutin |
| FC Sibiryak Bratsk | Valeri Tolchev |
| FC Selenga Ulan-Ude | Valeri Mikhnov |
| FC Amur-Energiya Blagoveshchensk | Vyacheslav Kurtsayev |
| FC Dynamo Barnaul | Viktor Volynkin |
| FC Kuzbass Kemerovo | Sergei Rogalevskiy, Boris Rusanov |
| FC Dynamo Omsk | Anatoli Chikinskiy |
Neftekhimik won 4–1 on aggregate and was promoted to the 2001 Russian First Division.----
3–3 on aggregate, Severstal won on away goals rule and was promoted to the 2001 Russian First Division. However, Severstal could not find necessary financing in time and the spot was eventually given to Khimki instead.----
Kuban Krasnodar won 1–0 on aggregate and was promoted to the 2001 Russian First Division.----