| Competition: | Primera B de Chile |
| Season: | 2000 |
| Winners: | Unión San Felipe |
| Promoted: | Unión San Felipe Rangers |
| Relegated: | Deportes Linares |
| League Topscorer: | Gustavo Biscayzacú (18) |
| Prevseason: | 1999 |
| Nextseason: | 2001 |
The 2000 Primera B de Chile was the 50th completed season of the Primera B de Chile.