| Year: | 2000 |
| Team: | Northern Iowa Panthers |
| Sport: | football |
| Conference: | Gateway Football Conference |
| Short Conf: | Gateway |
| Tsnrank: | 19 |
| Record: | 7–4 |
| Conf Record: | 3–3 |
| Head Coach: | Mike Dunbar |
| Hc Year: | 4th |
| Def Coach: | Jay Niemann |
| Dc Year: | 2nd |
| Stadium: | UNI-Dome |
The 2000 Northern Iowa Panthers football team represented the University of Northern Iowa in the 2000 NCAA Division I-AA football season as a member of the Gateway Football Conference. Led by Mike Dunbar in his fourth and final season as head coach, the Panthers compiled an overall record of 7–4 with a mark of 3–3 in conference play, placing fourth in the Gateway.