| Division: | II |
| Year: | 2000 |
| Gender: | Men's |
| Teams: | 48 |
| Finalfourcity: | Commonwealth Convention Center Louisville, Kentucky |
| Champions: | Metro State |
| Titlecount: | 1st |
| Runnerup: | Kentucky Wesleyan |
| Gamecount: | 10th |
| Semifinal1: | Missouri Southern |
| Finalfourcount: | 1st |
| Semifinal2: | Seattle Pacific |
| Finalfourcount2: | 5th |
| Coach: | Mike Dunlap |
| Coachcount: | 1st |
| Mop: | DeMarcos Anzures |
| Mopteam: | Metro State |
The 2000 NCAA Division II men's basketball tournament was the 44th annual single-elimination tournament to determine the national champion of men's NCAA Division II college basketball in the United States.
Officially culminating the 1999–2000 NCAA Division II men's basketball season, the tournament featured forty-eight teams from around the country.
The Elite Eight, national semifinals, and championship were again played at the Commonwealth Convention Center in Louisville, Kentucky.
In a rematch of the previous year's final, Metro State (33–4) defeated defending champions Kentucky Wesleyan, 97–79, to win their first Division II national championship.
The Roadrunners were coached by Mike Dunlap. Metro State's DeMarcos Anzures was the Most Outstanding Player.
Location: Jenkins Field House Host: Florida Southern College
Location: Leggett & Platt Athletic Center Host: Missouri Southern State University
Location: James Moore Fieldhouse Host: Southern Connecticut State University
Location: Auraria Events Center Host: Metropolitan State University
Location: Eddie King Gymnasium Host: University of Charleston
Location: Sportscenter Host: Kentucky Wesleyan College
Location: Cuddy Arena Host: Wingate University
Location: Royal Brougham Pavilion Host: Seattle Pacific University
Location: Commonwealth Convention Center Host: Bellarmine College