| Gender: | Women's |
| Year: | 2000 |
| Division: | III |
| Teams: | 49 |
| Finalfourcity: | Danbury, Connecticut |
| Finalfourarena: | O'Neill Center |
| Champions: | Washington University Bears |
| Titlecount: | 3rd |
| Runnerup: | Southern Maine Huskies |
| Gamecount: | 2nd |
| Thirdplace: | St. Thomas Tommies |
| Finalfourcount: | 4th |
| Fourthplace: | Scranton Royals |
| Finalfourcount2: | 6th |
| Coach: | Nancy Fahey |
| Coachcount: | 3rd |
| Mop: | Alia Fischer |
| Mopteam: | Washington University in St. Louis |
| Attendance: | 30465 |
The 2000 NCAA Division III women's basketball tournament was the 19th annual tournament hosted by the NCAA to determine the national champion of Division III women's collegiate basketball in the United States.[1]
Two-time defending champions Washington St. Louis defeated Southern Maine in the championship game, 79–33, to claim the Bears' third Division III national title, their third of four consecutive.
The championship rounds were hosted by Western Connecticut State University at the O'Neill Center in Danbury, Connecticut from March 17–18, 2000.