| Year: | 2000 |
| Division: | III |
| Teams: | 48 |
| Finalfourcity: | Salem, Virginia |
| Champions: | Calvin |
| Titlecount: | 2nd |
| Runnerup: | Wisconsin–Eau Claire |
| Gamecount: | 1st |
| Semifinal1: | Salem State |
| Finalfourcount: | 1st |
| Semifinal2: | Franklin & Marshall |
| Finalfourcount2: | 4th |
| Coach: | Kevin Vande Streek (Calvin) |
| Mop: | Sherm Carstensen (Wisconsin-Eau Claire) |
| Attendance: | 52847 |
The 2000 NCAA Division III men's basketball tournament was the 26th annual single-elimination tournament to determine the national champions of National Collegiate Athletic Association (NCAA) men's Division III collegiate basketball in the United States.
The field contained forty-eight teams, and each program was allocated to one of four sectionals. All sectional games were played on campus sites, while the national semifinals, third-place final, and championship finals were contested at the Salem Civic Center in Salem, Virginia.[1]
Calvin defeated Wisconsin–Eau Claire, 79–74, in the championship, clinching a second national title.
The Knights (31–1) were coached by Kevin Vande Streek. Calvin's prior championship came in 1992.
Sherm Carstensen of Wisconsin-Eau Claire was named Most Outstanding Player after scoring 34 points in the semifinal and 36 in the championship game.[2]