| Year: | 2000 |
| Team: | McNeese State Cowboys |
| Sport: | football |
| Conference: | Southland Conference |
| Short Conf: | Southland |
| Tsnrank: | 16 |
| Record: | 8–4 |
| Conf Record: | 5–2 |
| Head Coach: | Tommy Tate |
| Hc Year: | 1st |
| Off Coach: | Matt Viator |
| Oc Year: | 1st |
| Def Coach: | Scott Stoker |
| Dc Year: | 1st |
| Stadium: | Cowboy Stadium |
| Bowl Result: | L 17–42 vs. Georgia Southern |
The 2000 McNeese State Cowboys football team was an American football team that represented McNeese State University as a member of the Southland Conference (Southland) during the 2000 NCAA Division I-AA football season. In their first year under head coach Tommy Tate, the team compiled an overall record of 8–4, with a mark of 5–2 in conference play, and finished tied for second in the Southland. The Cowboys advanced to the NCAA Division I-AA Football Championship playoffs and lost to Georgia Southern in the first round.