| MLS All-Star Game 2000 | |
| Event: | 2000 Major League Soccer season |
| Team1: | MLS West |
| Team1score: | 4 |
| Team2: | MLS East |
| Team2score: | 9 |
| Date: | July 29, 2000 |
| Stadium: | Columbus Crew Stadium |
| City: | Columbus, Ohio |
| Man Of The Match1a: | Mamadou Diallo (MLS East) |
| Referee: | Paul Tamberino |
| Attendance: | 23,495 |
| Weather: | Cloudy, 78°F |
| Previous: | 1999 |
| Next: | 2001 |
The 2000 Major League Soccer All-Star Game was the fifth MLS All-Star Game. It was played on July 29, 2000 at Columbus Crew Stadium in Columbus, Ohio.[1] In the highest scoring MLS All-Star Game, the Eastern Conference won 9-4.
Players from the league's three division were divided into East and West teams, decided by fans through voting online and at stadiums and stores.[2]
| GK | ||||||
| DF | ||||||
| DF |  Greg Vanney | |||||
| DF | Marcelo Balboa | |||||
| DF | ||||||
| MF | Preki (c) | |||||
| MF | ||||||
| MF | 12 | |||||
| MF | ||||||
| FW | 9 | |||||
| FW | 13 | |||||
| Substitutions: | ||||||
| GK | 22 | |||||
| DF | ||||||
| MF | ||||||
| FW | ||||||
| MF | ||||||
| MF | 2 | |||||
| FW | ||||||
| FW | ||||||
| Manager: | ||||||
| GK | 1 | ||||
| DF | |||||
| DF | |||||
| DF | |||||
| DF | 6 | John Harkes | |||
| MF | |||||
| MF |  Carlos Valderrama | ||||
| MF | |||||
| MF | 13 | ||||
| FW | 9 | ||||
| FW | |||||
| Substitutions: | |||||
| GK | |||||
| DF | 33 | ||||
| DF | 4 | ||||
| DF | |||||
| FW | 20 | ||||
| MF | |||||
| FW | 16 | ||||
| FW | |||||
| Manager: | |||||
Assistant referees:
George Vergara
Robert Fereday