| Year: | 2000 |
| Team: | Louisville Cardinals |
| Conference: | Conference USA |
| Short Conf: | C-USA |
| Record: | 9–3 |
| Conf Record: | 6–1 |
| Hc Year: | 3rd |
| Oc Year: | 2nd |
| Def Coach: | Chris Smeland |
| Dc Year: | 3rd |
| Champion: | C-USA champion |
| Bowl Result: | L 17 - 22 vs. Colorado State |
The 2000 Louisville Cardinals football team represented the University of Louisville in the 2000 NCAA Division I-A football season. The team, led by John L. Smith and played their home games in Papa John's Cardinal Stadium, ended with a 9–3 record.
See also: 2000 NCAA Division I-A football rankings.