| Competition: | Japan Football League |
| Season: | 2000 |
| Dates: | 16 April – 12 November |
| Winners: | Yokohama FC 2nd JFL title 2nd D3 title |
| Promoted: | Yokohama FC |
| League Topscorer: | Kenji Arima (24 goals total) |
| Matches: | 132 |
| Total Goals: | 468 |
| Highest Attendance: | 5,324 (Round 1, Yokohama vs. Tochigi) |
| Lowest Attendance: | 84 (Round 17, Kokushinkan vs. ALO's) |
| Average Attendance: | 901 |
| Prevseason: | 1999 |
| Nextseason: | 2001 |
The was the second season of the Japan Football League, the third tier of the Japanese football league system.
It was contested by 12 teams, and Yokohama FC defended their champions title.
| Rank | Scorer | Club | Goals |
|---|---|---|---|
| 1 |  Kenji Arima | Yokohama FC | 24 |
| 2 | Hidenori Tabata | Sony Sendai | 19 |
| 3 | Takehiro Hayashi | Otsuka Pharmaceuticals | 16 |
| 4 | Shusuke Shimada | Otsuka Pharmaceuticals | 15 |
| 5 | Tatsuya Furuhashi | Honda Motors | 14 |
No relegation has occurred as league continued to expand. At the end of the season, the winner and runner-up of the Regional League promotion series, Sagawa Express Tokyo and YKK FC were promoted automatically. In addition, NTT West Kumamoto, SC Tottori and Ehime FC were included by JFA recommendation.