| Main Name: | Portugal Open |
| Champ: | Anke Huber |
| Runner: |  Nathalie Dechy |
| Score: | 6–2, 1–6, 7–5 |
| Before Name: | Estoril Open – Women's singles |
| After Name: | Estoril Open – Women's singles |
See main article: 2000 Estoril Open.
Katarina Srebotnik was the defending champion, but lost in first round to Seda Noorlander.
Anke Huber won the title by defeating Nathalie Dechy 6–2, 1–6, 7–5 in the final.