See main article: 2000 Ericsson Open.
Martina Hingis defeated Lindsay Davenport in the final, 6–3, 6–2 to win the women's singles tennis title at the 2000 Miami Open. She did not lose a set, and lost only 21 games, en route to the title.
Venus Williams was the two-time defending champion, but did not participate this year.
A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated. All thirty-two seeds received a bye to the second round.