See main article: 2000 Ericsson Open.
Pete Sampras defeated Gustavo Kuerten in the final, 6–1, 6–7(2–7), 7–6(7–5), 7–6(10–8) to win the men's singles tennis title at the 2000 Miami Open.
Richard Krajicek was the reigning champion, but did not participate this year.
All thirty-two seeds received a bye to the second round.