| Competition: | Serie A |
| Season: | 2000 |
| Winners: | Olmedo (1st title) |
| Relegated: | LDU Quito Técnico Universitario |
| Continentalcup1: | Copa Libertadores |
| Continentalcup1 Qualifiers: | Olmedo El Nacional Emelec |
| League Topscorer: | Alejandro Kenig (25 goals) |
| Prevseason: | 1999 |
| Nextseason: | 2001 |
The 2000 Campeonato Ecuatoriano de Fútbol de la Serie A was the 42nd season of the Serie A, the top level of professional football in Ecuador. The season was won by Olmedo, who became the first team outside of Quito and Guayaquil to win a national title.