| Competition: | Belarusian First League |
| Season: | 2000 |
| Winners: | Molodechno |
| Promoted: | Molodechno |
| Relegated: | Traktor Minsk Veino-Dnepr Polesye Kozenki |
| Matches: | 240 |
| Total Goals: | 704 |
| Prevseason: | 1999 |
| Nextseason: | 2001 |
2000 Belarusian First League was the tenth season of 2nd level football championship in Belarus. It started in April and ended in November 2000.
Two best teams of 1999 Belarusian First League (Kommunalnik Slonim and Vedrich-97 Rechitsa) were promoted to Belarusian Premier League. They were replaced by two lowest placed teams of 1999 Premier League (Molodechno and Svisloch-Krovlya Osipovichi).
Two lowest placed teams of the 1999 First League (Pinsk-900 and Vitbich-Dinamo-Energo Vitebsk) relegated to the Second League. They were replaced by two newcomers from the Second League (Traktor Minsk and Luninets as the winners of their respective groups).
Before the start of the season Kommunalnik Svetlogorsk were renamed to Khimik Svetlogorsk.
This season winners Molodechno were promoted to the Premier League. Four lowest placed teams (Traktor Minsk, Khimik Svetlogorsk, Veino-Dnepr and Polesye Kozenki) were initially relegated to the Second League, although Khimik Svetlogorsk later avoided relegation due to withdrawal of two other teams before 2001 season.
| width=120 | Team | width=110 | Location | data-sort-type="number" | Position in 1999 |
|---|---|---|---|---|---|
| Svisloch-Krovlya | Osipovichi | Premier League, 15 | |||
| Molodechno | Molodechno | Premier League, 16 | |||
| Dinamo-Juni | Minsk | 3 | |||
| Khimik | Svetlogorsk | 4 | |||
| Granit | Mikashevichi | 5 | |||
| Neman | Mosty | 6 | |||
| Torpedo | Zhodino | 7 | |||
| Keramik | Bereza | 8 | |||
| Orsha | Orsha | 9 | |||
| Rogachev | Rogachev | 10 | |||
| ZLiN | Gomel | 11 | |||
| Zvezda-VA-BGU | Minsk | 12 | |||
| Polesye | Kozenki | 13 | |||
| Veino-Dnepr | Veino | 14 | |||
| Traktor | Minsk | Second League, Group A, 1 | |||
| Luninets | Luninets | Second League, Group B, 1 |
| Rank | width=170 | Goalscorer | width=170 | Team | Goals |
|---|---|---|---|---|---|
| 1 | Keramik Bereza | 19 | |||
| 2 |  Nikolay Kurilovich | Keramik Bereza | 17 | ||
| 3 | Vladimir Taran | Torpedo Zhodino | 15 | ||
| 4 | Vladimir Provalinskiy | Orsha | 14 | ||
| Maksim Tsyhalka | Dinamo-Juni Minsk | 14 |